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Презентация на тему Transpositional site-specific recombination

Three of the many types of mobile genetic elements found in bacteriaTransposase gene: encoding enzymes for DNA breakage and joiningRed segments: DNA sequences as recognition sites for enzymesYellow segments: antibiotic genes
Transpositional site-specific recombinationModest target site selectivity and insert mobile genetic elements into Three of the many types of mobile genetic elements found in bacteriaTransposase Cut and Paste TranspositionDNA-only The structure of the central intermediate formed by transposase (integrase) Replicative Transposition Retrovirus-based TranspositionRetroviral-like retrotransposition Reverse TranscriptaseFrom RNA to DNA Non-retroviral retrotranspositionL1 Element Conservative Site Specific RecombinationIntegration vs. inversionNotice the arrows of directions Bacteriophase Lambda Genetic Engineering to control Gene expression SummaryDNA site-specific recombinationtranspositional; conservativeTransposons: mobile genetic elementsTranspositional: DNA only transposons, retroviral-like retrotransposons, nonretroviral retrotransposons RNA splicing reactions 3 Important sequences for Splicing to occurR: A or G; Y: C or U RNA Splicing mechanismBBP: branch-point binding proteinU2AF: a helper proteinsnRNA: small nuclear RNAsnRNP: Further mechanism to mark Exon and Intron differenceCBC: capping binding complexhnRNP: heterogeneous Consensus sequence for 3’ processAAUAAA: CstF (cleavage stimulation factor F)GU-rich sequence: CPSF The Genetic Code The Reading Frames tRNA (clover leaf shape with four strands folded, finally L-shape) tRNA and mRNA pairing
Слайды презентации

Слайд 2 Three of the many types of mobile genetic

Three of the many types of mobile genetic elements found in

elements found in bacteria

Transposase gene: encoding enzymes for DNA

breakage and joining
Red segments: DNA sequences as recognition sites for enzymes
Yellow segments: antibiotic genes

Слайд 4 Cut and Paste Transposition
DNA-only

Cut and Paste TranspositionDNA-only

Слайд 5 The structure of the central intermediate formed by

The structure of the central intermediate formed by transposase (integrase)

transposase (integrase)


Слайд 6 Replicative Transposition

Replicative Transposition

Слайд 7 Retrovirus-based Transposition
Retroviral-like retrotransposition

Retrovirus-based TranspositionRetroviral-like retrotransposition

Слайд 9 Reverse Transcriptase
From RNA to DNA

Reverse TranscriptaseFrom RNA to DNA

Слайд 10 Non-retroviral retrotransposition
L1 Element

Non-retroviral retrotranspositionL1 Element

Слайд 11 Conservative Site Specific Recombination
Integration vs. inversion
Notice the arrows

Conservative Site Specific RecombinationIntegration vs. inversionNotice the arrows of directions

of directions


Слайд 12 Bacteriophase Lambda

Bacteriophase Lambda

Слайд 13 Genetic Engineering to control Gene expression

Genetic Engineering to control Gene expression

Слайд 14 Summary

DNA site-specific recombination
transpositional; conservative
Transposons: mobile genetic elements
Transpositional: DNA

SummaryDNA site-specific recombinationtranspositional; conservativeTransposons: mobile genetic elementsTranspositional: DNA only transposons, retroviral-like retrotransposons, nonretroviral retrotransposons

only transposons, retroviral-like retrotransposons, nonretroviral retrotransposons


Слайд 16 RNA splicing reactions

RNA splicing reactions

Слайд 17 3 Important sequences for Splicing to occur
R: A

3 Important sequences for Splicing to occurR: A or G; Y: C or U

or G; Y: C or U


Слайд 18 RNA Splicing mechanism
BBP: branch-point binding protein
U2AF: a helper

RNA Splicing mechanismBBP: branch-point binding proteinU2AF: a helper proteinsnRNA: small nuclear

protein

snRNA: small nuclear RNA
snRNP: small nuclear ribonucleoprotein
Components for splicesome


Слайд 20 Further mechanism to mark Exon and Intron difference
CBC:

Further mechanism to mark Exon and Intron differenceCBC: capping binding complexhnRNP:

capping binding complex
hnRNP: heterogeneous nuclear ribonucleoprotein, binding to introns
SR:

rich in serine and arginines, binding to exons

Слайд 21 Consensus sequence for 3’ process
AAUAAA: CstF (cleavage stimulation

Consensus sequence for 3’ processAAUAAA: CstF (cleavage stimulation factor F)GU-rich sequence:

factor F)
GU-rich sequence: CPSF (cleavage and polyadenylation specificity factor)


Слайд 23 The Genetic Code

The Genetic Code

Слайд 24 The Reading Frames

The Reading Frames

Слайд 25 tRNA (clover leaf shape with four strands folded,

tRNA (clover leaf shape with four strands folded, finally L-shape)

finally L-shape)


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