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Презентация на тему Introduction to Enterobacteriaceae

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Introduction to Enterobacteriaceae
Introduction to Enterobacteriaceae EnterobacteriaceaeSmall gram-negative rods (2-5 by 0.5 microns)Most motile with peritrichous flagella Shigella Ferment glucose Reduce nitratesNO3 to NO2 or all the way to N2 Gram-Positive Cell Wall Gram-Negative Cell Wall Antigenic Structure of EnterobacteriaceaeS. typhiO antigen side chain(Fimbriae) Diversity of Activities Associated with LPS ENDOTOXIN1. Integral part of cell wall2. Endotoxin is LPS; Lipid A is Structure of Lipopolysaccharide Structure of Lipid A Hydrophobic Lipid A is endotoxic component Structure of Core Polysaccharide KDO is distinctive sugar moiety in core polysaccharide Repeat Units of O Antigen Side Chain Heat stable O antigen is often used to serotype Taxonomic Descriptions of Prokaryotes Based   on Numbers and Arrangements of Flagella Polar       		Monotrichous, a single flagellum at Escherichia coli Hypermotile Swarming growthProteus vulgaris Family Enterobacteriaceae(nonmotile)(nonmotile)True pathogenTrue pathogenTrue pathogenCertain E .coli strains can be considered   true pathogens Citrobacter speciesEnterobacter spp.Escherichia spp.Klebsiella spp.Morganella spp.Proteus spp.Salmonella spp.Serratia spp.Shigella spp.Yersinia spp.Medically Important Enterobacteriaceae Incidence of Enterobacteriaceae Associated with Bacteremia Sites of Infections with Members of the Enterobacteriaceae DNA Relatedness Among Common Enterobacteriaceae REVIEW Ferment glucose Reduce nitratesNO3 to NO2 or all the way to N2 Gram-Negative Cell WallREVIEW Antigenic Structure of EnterobacteriaceaeS. typhiO antigen side chain(Fimbriae)REVIEW ENDOTOXIN1. Integral part of cell wall2. Endotoxin is LPS; Lipid A is Structure of LipopolysaccharideREVIEW Taxonomic Descriptions of Prokaryotes Based   on Numbers and Arrangements of FlagellaREVIEW Family Enterobacteriaceae(nonmotile)(nonmotile)True pathogenTrue pathogenTrue pathogenCertain E .coli strains can be considered   true pathogensREVIEW Sites of Infections with Members of the EnterobacteriaceaeREVIEW
Слайды презентации

Слайд 2 Introduction to Enterobacteriaceae


Introduction to Enterobacteriaceae

Слайд 3 Enterobacteriaceae
Small gram-negative rods (2-5 by 0.5 microns)
Most motile

EnterobacteriaceaeSmall gram-negative rods (2-5 by 0.5 microns)Most motile with peritrichous flagella

with peritrichous flagella
Shigella and Klebsiella are nonmotile
Oxidase-negative facultative

anaerobes
Reduce nitrate
Ferment glucose and other carbohydrates
Many genera
Escherichia, Salmonella, Shigella, Klebsiella, Proteus, Enterobacter, Yersinia, etc.
Some strains opportunistic pathogens
Some strains true pathogens
Salmonella, Shigella, Yersinia, some strains of E. coli

Слайд 4
Ferment glucose
Reduce nitrates
NO3 to NO2 or all

Ferment glucose Reduce nitratesNO3 to NO2 or all the way to

the way to N2
Oxidase negative
Distinguishing Properties Associated

with All Enterobacteriaceae:

Слайд 5 Gram-Positive Cell Wall

Gram-Positive Cell Wall

Слайд 6 Gram-Negative Cell Wall

Gram-Negative Cell Wall

Слайд 7 Antigenic Structure of Enterobacteriaceae
S. typhi
O antigen side chain
(Fimbriae)

Antigenic Structure of EnterobacteriaceaeS. typhiO antigen side chain(Fimbriae)

Слайд 9 Diversity of Activities Associated with LPS

Diversity of Activities Associated with LPS

Слайд 10 ENDOTOXIN

1. Integral part of cell wall


2. Endotoxin is

ENDOTOXIN1. Integral part of cell wall2. Endotoxin is LPS; Lipid A

LPS; Lipid A is toxic component

3. Heat

stable

4. Antigenic; ??immunogenicity

5. Toxoids cannot be produced

6. Many effects on host

7. Produced by gram-negative organisms only

EXOTOXIN

1. Released from the cell before
or after lysis

2. Protein

3. Heat labile

4. Antigenic and immunogenic
5. Toxoids can be produced

6. Specific in effect on host

7. Produced by gram-positive and gram-negative organisms


Слайд 11 Structure of Lipopolysaccharide

Structure of Lipopolysaccharide

Слайд 12 Structure of Lipid A
Hydrophobic Lipid A is

Structure of Lipid A Hydrophobic Lipid A is endotoxic component

endotoxic component


Слайд 13 Structure of Core Polysaccharide
KDO is distinctive sugar

Structure of Core Polysaccharide KDO is distinctive sugar moiety in core polysaccharide

moiety in core polysaccharide


Слайд 14 Repeat Units of O Antigen Side Chain
Heat

Repeat Units of O Antigen Side Chain Heat stable O antigen is often used to serotype

stable O antigen is often used to serotype


Слайд 15 Taxonomic Descriptions of Prokaryotes Based on

Taxonomic Descriptions of Prokaryotes Based  on Numbers and Arrangements of Flagella

Numbers and Arrangements of Flagella


Слайд 16 Polar Monotrichous,

Polar    		Monotrichous, a single flagellum at one 				or

a single flagellum at one
or both ends of

the cell
Multitrichous, two or several flagella at one
or both ends of the cell
Lateral Flagella arise predominantly from the
middle pole of the cell
Monotrichous, one flagellum
Multitrichous, several flagella in the form
of a tuft originating from the midportion
of the cell
Peritrichous Random, haphazard arrangement of
flagella scattered around the bacterial cell
Mixed Two or more flagella exhibiting distinctly
different physical properties in different regions of the bacterial cell

Taxonomic Descriptions of Prokaryotes Based on Numbers and Arrangements of Flagella (cont.)


Слайд 17 Escherichia coli
Hypermotile
Swarming growth


Proteus vulgaris

Escherichia coli Hypermotile Swarming growthProteus vulgaris

Слайд 18 Family Enterobacteriaceae
(nonmotile)
(nonmotile)
True pathogen
True pathogen
True pathogen
Certain E .coli strains

Family Enterobacteriaceae(nonmotile)(nonmotile)True pathogenTrue pathogenTrue pathogenCertain E .coli strains can be considered  true pathogens

can be considered true pathogens


Слайд 19 Citrobacter species

Enterobacter spp.

Escherichia spp.

Klebsiella spp.

Morganella spp.

Proteus spp.

Salmonella spp.

Serratia

Citrobacter speciesEnterobacter spp.Escherichia spp.Klebsiella spp.Morganella spp.Proteus spp.Salmonella spp.Serratia spp.Shigella spp.Yersinia spp.Medically Important Enterobacteriaceae

spp.

Shigella spp.

Yersinia spp.
Medically Important Enterobacteriaceae


Слайд 20 Incidence of Enterobacteriaceae Associated with Bacteremia

Incidence of Enterobacteriaceae Associated with Bacteremia

Слайд 21 Sites of Infections with Members of the Enterobacteriaceae

Sites of Infections with Members of the Enterobacteriaceae

Слайд 22 DNA Relatedness Among Common Enterobacteriaceae

DNA Relatedness Among Common Enterobacteriaceae

Слайд 24 REVIEW

REVIEW

Слайд 25
Ferment glucose
Reduce nitrates
NO3 to NO2 or all

Ferment glucose Reduce nitratesNO3 to NO2 or all the way to

the way to N2
Oxidase negative
Distinguishing Properties Associated

with All Enterobacteriaciae:

REVIEW


Слайд 26 Gram-Negative Cell Wall
REVIEW

Gram-Negative Cell WallREVIEW

Слайд 27 Antigenic Structure of Enterobacteriaceae
S. typhi
O antigen side chain
(Fimbriae)
REVIEW

Antigenic Structure of EnterobacteriaceaeS. typhiO antigen side chain(Fimbriae)REVIEW

Слайд 28 ENDOTOXIN

1. Integral part of cell wall


2. Endotoxin is

ENDOTOXIN1. Integral part of cell wall2. Endotoxin is LPS; Lipid A

LPS; Lipid A is toxic component

3. Heat

stable

4. Antigenic; ??immunogenicity

5. Toxoids cannot be produced

6. Many effects on host

7. Produced by gram-negative organisms only

EXOTOXIN

1. Released from the cell before
or after lysis

2. Protein

3. Heat labile

4. Antigenic and immunogenic
5. Toxoids can be produced

6. Specific in effect on host

7. Produced by gram-positive and gram-negative organisms

REVIEW


Слайд 29 Structure of Lipopolysaccharide
REVIEW

Structure of LipopolysaccharideREVIEW

Слайд 30 Taxonomic Descriptions of Prokaryotes Based on

Taxonomic Descriptions of Prokaryotes Based  on Numbers and Arrangements of FlagellaREVIEW

Numbers and Arrangements of Flagella
REVIEW


Слайд 31 Family Enterobacteriaceae
(nonmotile)
(nonmotile)
True pathogen
True pathogen
True pathogen
Certain E .coli strains

Family Enterobacteriaceae(nonmotile)(nonmotile)True pathogenTrue pathogenTrue pathogenCertain E .coli strains can be considered  true pathogensREVIEW

can be considered true pathogens
REVIEW


Слайд 32 Sites of Infections with Members of the Enterobacteriaceae
REVIEW

Sites of Infections with Members of the EnterobacteriaceaeREVIEW

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