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Презентация на тему Genome Editing

CRISPR Clustered Regularly Interspaced Short Palindromic Repeats Короткие палиндромные кластерные повторы, или CRISPR
Genome Editing CRISPR Clustered Regularly Interspaced Short Palindromic Repeats  Короткие палиндромные кластерные повторы, или CRISPR Two major domainsRuvCHNH Variety of CRISPR system CRISPR design tool W.-H. Wu et al., 2016. CRISPR repair reveals causative mutation in a K. A. Schaefer et al., 2017. Unexpected mutations after CRISPR-Cas9 editing in vivo117 insertions/delitions1397 SNP TALEN transcription activator-like nuclease From Xanthomonas genus – crops pathogen TALE proteins are composed of a central domain responsible for DNA binding, DNA-binding domain was demonstrated to consist of monomers, each of them binds
Слайды презентации

Слайд 2 CRISPR Clustered Regularly Interspaced Short Palindromic Repeats Короткие палиндромные

CRISPR Clustered Regularly Interspaced Short Palindromic Repeats Короткие палиндромные кластерные повторы, или CRISPR

кластерные повторы, или CRISPR


Слайд 12 Two major domains

RuvC
HNH

Two major domainsRuvCHNH

Слайд 15 Variety of CRISPR system

Variety of CRISPR system

Слайд 16 CRISPR design tool

CRISPR design tool

Слайд 17 W.-H. Wu et al., 2016. CRISPR repair reveals

W.-H. Wu et al., 2016. CRISPR repair reveals causative mutation in

causative mutation in a preclinical model of retinitis pigmentosa.


Слайд 18 K. A. Schaefer et al., 2017. Unexpected mutations

K. A. Schaefer et al., 2017. Unexpected mutations after CRISPR-Cas9 editing in vivo117 insertions/delitions1397 SNP

after CRISPR-Cas9 editing in vivo
117 insertions/delitions
1397 SNP


Слайд 19 TALEN transcription activator-like nuclease

TALEN transcription activator-like nuclease

Слайд 20 From Xanthomonas genus – crops pathogen

From Xanthomonas genus – crops pathogen

Слайд 21 TALE proteins are composed of a central domain

TALE proteins are composed of a central domain responsible for DNA

responsible for DNA binding, a nuclear localization signal, and

a domain that activates the target gene transcription


Слайд 22 DNA-binding domain was demonstrated to consist of monomers,

DNA-binding domain was demonstrated to consist of monomers, each of them

each of them binds one nucleotide in the target

nucleotide sequence

Monomers are tandem repeats of 34 amino acid residues, two of which are located at positions 12 and 13 and are highly variable (repeat variable diresidue, RVD), and it is they that are responsible for the recognition of a specific nucleotide


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