Презентация на тему Микоплазмы и уреаплазмы

всех штаммов постянно -только 2
Гидрофильные белки 30000 - 200000

Да легко вымываются при изменении рН и ионной силы
набор АГ цитоплазмы постоянный

U.urealyticum
Различают 16 сероваров,
группы А и В
АГ-детерминанты: полипетиды мембраны
Типировать невозможно - многие серовары реагируют перекрестно,
У одного пациента - несколько сероваров

человека
Фосфолипаза
РНКазы, ДНКазы
Нейроменидаза
ADP-ribosylating and vacuolating cytotoxin of Mycoplasma pneumoniae represents

unique virulence determinant among bacterial pathogens
T. R. Kannan and Joel B. Baseman *

pneumoniae (MP) and Chlamydia pneumoniae (CP) bodies. Elementary bodies in the myocardial

fiber from a mouse of the MP + CP infected group. The close view on the left side shows the double membrane of CP elementary bodies (1A). An intimal plaque from a mouse of the MP infected group, exhibiting two rounded mycoplasma bodies, characterized by only one envelopment membrane (1B).
Damy et al. BMC Microbiology 2009 9:194   doi:10.1186/1471-2180-9-194

http://www.biomedcentral.com/1471-2180/9/194/figure/F1?highres=y

of sperm incubated in vitro with M.genitalium. A single cell of M.genitaliumis attached

to (A) the midpiece region (arrow) and (B) the tail (arrow) of spermatozoa. (C) Several cells of M.genitalium attached to the head and midpiece. Note the swollen midpiece, which was not a general observation when spermatozoa with adhering mycoplasmas were studied. (D) Microcolonies of M.genitalium attached to the distal tail, mid‐tail, midpiece and head of the spermatozoon. (E) A massive colonization of the spermatozoon. Note that M.genitalium is also attached to the invisible head‐vesicle of the spermatozoon. (F) A negative control of sperm incubated without mycoplasmas. Bar = 5 µm.

http://humrep.oxfordjournals.org/content/18/10/2103/F1.expansion

observed at high magnification (≥8400×).
Spermatozoa were analyzed at greater

than or equal to 8400x magnification by inverted microscope equipped with DIC/Nomarski differential interference contrast optics. At least 200 motile spermatozoa per semen sample were evaluated and percentages of normal spermatozoa and spermatozoa with large nuclear vacuoles (LNV/one or more vacuoles occupying>50% of the sperm nuclear area) were determined. A spermatozoon was classified as morphologically normal when it exhibited a normal nucleus (smooth, symmetric and oval nucleus, width 3.28+/-0.20 microm, length 4.75+/-0.20 microm/absence of vacuoles occupying>4% of nuclear area) as well as acrosome, post-acrosomal lamina, neck and tail, besides not presenting cytoplasm around the head. One examiner, blinded to subject identity, performed the entire study.

http://php.med.unsw.edu.au/embryology/index.php?title=File:Human-_vacuolated_spermatozoa.jpg

зависимости от наличия и вида генитальных микоплазм

Микроскопия окрашенных препаратов, бак. посев;
C.trachomatis. РИФ, серологический анализ, бак.посев;
T.vaginalis.

Микроскопия нативных и окрашенных перпаратов, микробиол. посев;
Mycoplasma genitalium - отсутствуют

в результате чего в большинстве случаев этиология инфекции остается

неустановленной. Пример – пациенты с уретритом:

«не-ПЦР»

«ПЦР»

Ширшова Е.В., Кисина В.И., Гущин А.Е., 2007